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spag6 polyclonal antibody  (Bioss)


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    Bioss spag6 polyclonal antibody
    Spag6 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+spag6+antibody/bioss___bs-12291r?v=Bioss
    Average 94 stars, based on 5 article reviews
    spag6 polyclonal antibody - by Bioz Stars, 2026-07
    94/100 stars

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    Sperm morphology, ultrastructural deficiency, and immunofluorescence staining in individuals carrying biallelic DNAH12 variants (A and B) The morphology of spermatozoa from proband AY0749 and XM0178 under light microscopy and scanning electron microscopy (SEM). A: Scale bar, 10μm; B: Scale bar, 2μm. (C) Abnormal sperm flagella ultrastructure was observed in proband AY0749 compared to normal spermatozoa by transmission electron microscopy (TEM), including the absence of central pair apparatus. Scale bar, 500nm. (D) Spermatozoa from normal male control and AY0749 harboring bi-allelic DNAH12 variants were stained with anti-DNAN12 (red) and anti-Ac-tubulin (green) antibodies. A notable absence of the DNAH12 signal was revealed in the flagella of AY0749 spermatozoa. Scale bar, 10μm. (E) DNAH1 signal (red) was present in both control and AY0749. Scale bar, 10μm. Scale bar, 10μm. (F) <t>SPAG6</t> signal (red) was present normally in the spermatozoa from control, while absent in proband AY0749. Scale bar, 10μm. (G) DNAI2 signal (red) was present in both control and AY0749. Scale bar, 10μm. NC, normal control; CP, central pair apparatus (green arrows); DMT, peripheral microtubule doublet (blue arrows); ODF, outer dense fiber (red arrows); MS, mitochondrial sheath (yellow arrows).
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    Sperm morphology, ultrastructural deficiency, and immunofluorescence staining in individuals carrying biallelic DNAH12 variants (A and B) The morphology of spermatozoa from proband AY0749 and XM0178 under light microscopy and scanning electron microscopy (SEM). A: Scale bar, 10μm; B: Scale bar, 2μm. (C) Abnormal sperm flagella ultrastructure was observed in proband AY0749 compared to normal spermatozoa by transmission electron microscopy (TEM), including the absence of central pair apparatus. Scale bar, 500nm. (D) Spermatozoa from normal male control and AY0749 harboring bi-allelic DNAH12 variants were stained with anti-DNAN12 (red) and anti-Ac-tubulin (green) antibodies. A notable absence of the DNAH12 signal was revealed in the flagella of AY0749 spermatozoa. Scale bar, 10μm. (E) DNAH1 signal (red) was present in both control and AY0749. Scale bar, 10μm. Scale bar, 10μm. (F) <t>SPAG6</t> signal (red) was present normally in the spermatozoa from control, while absent in proband AY0749. Scale bar, 10μm. (G) DNAI2 signal (red) was present in both control and AY0749. Scale bar, 10μm. NC, normal control; CP, central pair apparatus (green arrows); DMT, peripheral microtubule doublet (blue arrows); ODF, outer dense fiber (red arrows); MS, mitochondrial sheath (yellow arrows).
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    Sperm morphology, ultrastructural deficiency, and immunofluorescence staining in individuals carrying biallelic DNAH12 variants (A and B) The morphology of spermatozoa from proband AY0749 and XM0178 under light microscopy and scanning electron microscopy (SEM). A: Scale bar, 10μm; B: Scale bar, 2μm. (C) Abnormal sperm flagella ultrastructure was observed in proband AY0749 compared to normal spermatozoa by transmission electron microscopy (TEM), including the absence of central pair apparatus. Scale bar, 500nm. (D) Spermatozoa from normal male control and AY0749 harboring bi-allelic DNAH12 variants were stained with anti-DNAN12 (red) and anti-Ac-tubulin (green) antibodies. A notable absence of the DNAH12 signal was revealed in the flagella of AY0749 spermatozoa. Scale bar, 10μm. (E) DNAH1 signal (red) was present in both control and AY0749. Scale bar, 10μm. Scale bar, 10μm. (F) <t>SPAG6</t> signal (red) was present normally in the spermatozoa from control, while absent in proband AY0749. Scale bar, 10μm. (G) DNAI2 signal (red) was present in both control and AY0749. Scale bar, 10μm. NC, normal control; CP, central pair apparatus (green arrows); DMT, peripheral microtubule doublet (blue arrows); ODF, outer dense fiber (red arrows); MS, mitochondrial sheath (yellow arrows).
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    Sperm morphology, ultrastructural deficiency, and immunofluorescence staining in individuals carrying biallelic DNAH12 variants (A and B) The morphology of spermatozoa from proband AY0749 and XM0178 under light microscopy and scanning electron microscopy (SEM). A: Scale bar, 10μm; B: Scale bar, 2μm. (C) Abnormal sperm flagella ultrastructure was observed in proband AY0749 compared to normal spermatozoa by transmission electron microscopy (TEM), including the absence of central pair apparatus. Scale bar, 500nm. (D) Spermatozoa from normal male control and AY0749 harboring bi-allelic DNAH12 variants were stained with anti-DNAN12 (red) and anti-Ac-tubulin (green) antibodies. A notable absence of the DNAH12 signal was revealed in the flagella of AY0749 spermatozoa. Scale bar, 10μm. (E) DNAH1 signal (red) was present in both control and AY0749. Scale bar, 10μm. Scale bar, 10μm. (F) SPAG6 signal (red) was present normally in the spermatozoa from control, while absent in proband AY0749. Scale bar, 10μm. (G) DNAI2 signal (red) was present in both control and AY0749. Scale bar, 10μm. NC, normal control; CP, central pair apparatus (green arrows); DMT, peripheral microtubule doublet (blue arrows); ODF, outer dense fiber (red arrows); MS, mitochondrial sheath (yellow arrows).

    Journal: iScience

    Article Title: Further evidence from DNAH12 supports favorable fertility outcomes of infertile males with dynein axonemal heavy chain gene family variants

    doi: 10.1016/j.isci.2024.110366

    Figure Lengend Snippet: Sperm morphology, ultrastructural deficiency, and immunofluorescence staining in individuals carrying biallelic DNAH12 variants (A and B) The morphology of spermatozoa from proband AY0749 and XM0178 under light microscopy and scanning electron microscopy (SEM). A: Scale bar, 10μm; B: Scale bar, 2μm. (C) Abnormal sperm flagella ultrastructure was observed in proband AY0749 compared to normal spermatozoa by transmission electron microscopy (TEM), including the absence of central pair apparatus. Scale bar, 500nm. (D) Spermatozoa from normal male control and AY0749 harboring bi-allelic DNAH12 variants were stained with anti-DNAN12 (red) and anti-Ac-tubulin (green) antibodies. A notable absence of the DNAH12 signal was revealed in the flagella of AY0749 spermatozoa. Scale bar, 10μm. (E) DNAH1 signal (red) was present in both control and AY0749. Scale bar, 10μm. Scale bar, 10μm. (F) SPAG6 signal (red) was present normally in the spermatozoa from control, while absent in proband AY0749. Scale bar, 10μm. (G) DNAI2 signal (red) was present in both control and AY0749. Scale bar, 10μm. NC, normal control; CP, central pair apparatus (green arrows); DMT, peripheral microtubule doublet (blue arrows); ODF, outer dense fiber (red arrows); MS, mitochondrial sheath (yellow arrows).

    Article Snippet: Anti-SPAG6 antibody (rabbit) , Sigma Aldrich , Cat#HPA038440; RRID: AB_10671742.

    Techniques: Immunofluorescence, Staining, Light Microscopy, Electron Microscopy, Transmission Assay, Control

    Journal: iScience

    Article Title: Further evidence from DNAH12 supports favorable fertility outcomes of infertile males with dynein axonemal heavy chain gene family variants

    doi: 10.1016/j.isci.2024.110366

    Figure Lengend Snippet:

    Article Snippet: Anti-SPAG6 antibody (rabbit) , Sigma Aldrich , Cat#HPA038440; RRID: AB_10671742.

    Techniques: Recombinant, Lysis, Protease Inhibitor, Staining, Software, Mutagenesis, Sequencing